Welcome
I am
Sebastián
Anibal Muñoz
Scientist.
PhD in Biological Chemistry - Microbiologist
Education
PhD in Biological Chemistry
2016-March 2022. Postgraduate thesis: “Nitrogen metabolism in Saccharomyces cerevisiae: molecular mechanisms that determine the hierarchy in the use of poor nitrogen sources”
Director: Dr. Bermúdez, Mariana. Department of Biological Chemistry, University of Buenos
Aires, Argentina. IQUIBICEN-CONICET
Bachelor of Science in Microbiology
2008-2014. Microbiology and Immunology Department, School of Applied/Natural Sciences
and Physical Chemistry, National University of Río Cuarto, Córdoba, Argentina. Grade thesis:
“Diversity of Aspergillus section Nigri isolated from low water activity food”. Director: Dr.
Reynoso, María Marta. Co-director: Dr.Giaj-Merlera, Guillermo.
Languages
Spanish – native language.
English – good listening and written comprehension.
Core Skills
Curiosity.
Organization.
Quick learning.
Good communication skills.
Laboratory management.
Scholarship
CONICET PhD scholarship. March 2016-March 2022. Laboratorio de regulación génica en
levaduras. Department of Biological Chemistry, University of Buenos Aires. IQUIBICEN-CONICET.
Advanced studies scholarship. 2007-2014. National University of Río Cuarto, Córdoba, Argentina.
Postgraduate
Courses
- TÓPICOS EN BIOTECNOLOGÍA BACTERIANA AMBIENTAL. Dpto. Química Biológica-FCEyN- UBA. March,2019
- APLICACIONES DE BACTERIAS A LA BIOTECNOLOGÍA INDUSTRIAL. Dpto. Química Biológica-FCEyN - UBA. February, 2019
- TÉCNICAS ELECTROFORÉTICAS. FUNDAMENTOS Y APLICACIONES. Dpto. Química Biológica-FCEyN - UBA. August, 2017
- MICROSCOPÍA ÓPTICA Y DE FLUORESCENCIA. DQB-FCEyN- UBA. June, 2017
- HERRAMIENTAS INFORMÁTICAS PARA EL ANÁLISIS ESTRUCTURAL DE ÁCIDOS NUCLEICOS Y PROTEÍNAS.
Dpto. Química Biológica-FCEyN- UBA. June 2017.
- TÓPICOS DE ESPECTROMETRÍA DE MASAS. Dpto. Química Orgánica – FCEyN – UBA. June, 2017
- GENÉTICA MOLECULAR DE LEVADURAS.
FBMC-FCEyN- UBA-Fundación Instituto Leloir.
October, 2016.
Other courses
“Sensibilización sobre los Sistemas de gestión de inocuidad de los alimentos”.
APMRA, November, 2012.
“Sensibilización sobre la serie de normas IRAM-ISO 9000”.
APMRA. June, 2012.
Professional
Experience
Scientist. Abalone Bio Inc. 2022-currently. Molecular biology and genetic engineering in yeast using CRISPR-Cas9 technology. Site-directed mutagenesis for plasmids and strains. Yeast transformation by LiCl-thermal shock. Mutant screening. Preparation of genomic and plasmid DNA. Experimental design. Primers design. Agarose gel electrophoresis. Laboratory management.
Doctoral research tasks applying various molecular biology techniques in yeasts.
2016-2022. Site-directed mutagenesis for plasmids and strains preparation expressing cMyc, HA or LacZ tagg fusion proteins. Yeast transformation by LiCl-thermal shock. Mutant screening.
Preparation of genomic and plasmid DNA. RNA extraction. Primers design. Agarose gel electrophoresis. ChIP. RT. qPCR. Immunoprecipitation. Protein extraction. Western blot
Head of HPLC chromatography area – SGS. 2015. Alejandro Roca. Córdoba, Argentina. Fumonisins extraction from peanut slurry and HPLC quantification. Task development certifying ISO and GLP standards.
Bachelor`s thesis research tasks applying various molecular biology and biochemical techniques in Aspergillus. 2012-2014. DNA preparation. Morphological identification through ISSR molecular markers. Ochratoxin A extraction and HPLC quantification.
Laboratory professor of Microbiology (Cod.3070) and Immunology (Cod.3077) at the Veterinary Medicine course of studies. August 2013-July 2014. Second grade assistant position. Microbiology and Immunology Department, School of Applied/Natural Sciences and Physical Chemistry, National University of Río Cuarto, Córdoba, Argentina. Resolution Nº: 173/2013.
Project collaborator on innovation and research for the improvement of grade teaching. 2013-2014. Microbiology and Immunology Department, School of Applied/Natural Sciences
and Physical Chemistry, National University of Río Cuarto, Córdoba, Argentina.
Research practice intern. 2012. On “Diversity of Aspergillus section Nigri species isolated from soybeans grown in different regions of Argentina”. Microbiology and Immunology Department, School of Applied/Natural Sciences and Physical Chemistry, National University of Río Cuarto, Córdoba, Argentina. Director: Mic. Giaj-Merlera, Guillermo. Resolution Nº: 190/12.
Scientific
Background
Publications
Novel function of transcription factor Uga3 as an activator of branched-chain amino acid
permease BAP2 gene expression. S.A. Muñoz , J.F. Gulias, J. Valencia-Guillén, S. Correa-García, M. Bermúdez-Moretti.
2019. Microbiology 166, 85-92.
Monitoring changes in the cellular content of biomolecules during ageing with FTIR spectroscopy. M. Bermúdez-Moretti , J.F. Gulías, J. Valencia-Guillén, S.A. Muñoz, I. Forfar, S. Correa-García. 2019. Vibrational spectroscopy, 105.
Diversity of black Aspergilli isolated from raisins in Argentina: Polyphasic approach to species
identification and development of SCAR markers for Aspergillus ibericus. G. Giaj Merlera,
S.A.Muñoz, I. Coelho, L.R. Cavaglieri, A.M. Torres, M.M. Reynoso. 2015. International Journal of
Food Microbiology 210, 92-101.
Conference presentations
“Novel function of transcription factor Uga3 as an activator of branched-chain amino acid
permease BAP2 gene expression”
Muñoz Sebastian, Gulías J, Valencia-Guillen J, Correa-García S and Bermúdez-Moretti M. JOINT
LV Annual SAIB meeting and XIV PABMB conference. SALTA, November 5 to 8, 2019.
“Dal81 and Uga3 transcription factors and stress response in Saccharomyces cerevisiae”;
Muñoz Sebastian, Mercau M, Gulías J, Valencia-Guillen J, Correa-García S and Bermúdez-
Moretti M. LIV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología
Molecular. Paraná, November 5 to 8, 2018.
Oral conference.
“Discovering new functions of GABA-responsive transcription factors in S. cerevisiae”
Sebastián Aníbal Muñoz, Juan Facundo Gulías, Jenniffer Valencia-Guillen, Susana Correa-
García, Mariana Bermúdez-Moretti. Reunión Conjunta de Sociedades de Biociencias: LIII
Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología
Molecular.CABA, November 13 to 17, 2017. Oral conference.
“Evaluación de la diversidad genética de Aspergillus sección Nigri aislados de soja y maní
utilizando marcadores ISSR”.
Giaj-Merlera, G. ; Muñoz, Sebastian A. ; Fantini, E. ; Palacios, S.A. ; Torres, A.M. ; Reynoso
M.M.. VII Congreso Latinoamericano de micotoxicología. Río Cuarto, Córdoba. Argentina.
December 3 to 6, 2013.
Conferences and seminars Attendance
“La contaminación de alimentos produce enfermedades” APMRA. 15/09/2011.
“Jornadas de higiene y seguridad” Área de seguridad y medio ambiente de la secretaria técnica
de la FCEFQyN de la UNRC. 12/12/012.
“VII congreso latinoamericano de micotoxicología”. 3-6/12/2013.
Contact
Find me
Sebastián Anibal Muñoz - 2023
